The "Whole Cell" Patch Clamp Technique

question 5

Multiple Choice

The "whole cell" patch clamp technique

• A) allows the experimenter to easily change the solution bathing the inside surface of the cell during the recording and study the activity of single voltage-gated channel currents.
• B) allows the experimenter to easily change the solution bathing the outside surface of the cell during the recording and study the activity of single voltage-gated channel currents.
• C) does not allow the experimenter to change the solution on either side of the cell, but you can study the summed activity of all voltage-gated channels in the cell (macroscopic current).
• D) allows the experimenter to easily change the solution bathing the inside surface of the cell during the recording and study the summed activity of all voltage-gated channels in the cell (macroscopic current).
• E) allows the experimenter to easily change the solution bathing the outside surface of the cell during the recording and study the summed activity of all voltage-gated channels in the cell (macroscopic current).

Understand and apply concepts of currency conversion and exchange rates.

Apply ratio and proportion knowledge to calculate overhead allocations and refund amounts.

Evaluate and solve problems involving additional investments to maintain original investment ratios.

Calculate the required increase in resources to meet specified ratios.

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Definitions:

RNA Primer

The sequence of about five RNA nucleotides that are synthesized during DNA replication to provide a 3' end to which DNA polymerase adds nucleotides. The RNA primer is later degraded and replaced with DNA.

RNA Primase

An enzyme that creates a short RNA primer as the first step in DNA replication, necessary for DNA polymerase to initiate synthesis.

Okazaki Fragments

Short sequences of DNA nucleotides which are synthesized discontinuously and later joined together during DNA replication on the lagging strand.

DNA Ligase

A catalyst that aids in connecting strands of DNA by promoting the creation of a phosphodiester linkage.