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Indicate the Correct Order of Steps for the Production of Monoclonal

question 23

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Indicate the correct order of steps for the production of monoclonal antibodies.
1) Hybridomas transferred to small wells and tested for antigen reactivity
2) B cells are harvested from the spleen of a mouse injected with antigen
3) Mixture of B cells, myeloma cells, and hybridomas are transferred to media that promotes only hybridoma survival
4) Long-lived hybridomas are grown in culture and their monoclonal antibodies collected for use
5) B cells mixed with multiple myeloma cells in special growth media to encourage hybridoma formation

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