Indicate the Correct Order of Steps for the Production of Monoclonal

question 23

Multiple Choice

Indicate the correct order of steps for the production of monoclonal antibodies.
1) Hybridomas transferred to small wells and tested for antigen reactivity
2) B cells are harvested from the spleen of a mouse injected with antigen
3) Mixture of B cells, myeloma cells, and hybridomas are transferred to media that promotes only hybridoma survival
4) Long-lived hybridomas are grown in culture and their monoclonal antibodies collected for use
5) B cells mixed with multiple myeloma cells in special growth media to encourage hybridoma formation

• A) 1, 2, 3, 4, 5
• B) 2, 5, 3, 1, 4
• C) 2, 3, 5, 1, 4
• D) 3, 5, 2, 4, 1

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