What Is the Correct Order of Steps in the Process

question 48

Multiple Choice

What is the correct order of steps in the process of cloning a Eukaryotic gene and inserting it into a plasmid?
1) isolate the spliced mRNA that produces the desired protein
2) synthesize a cDNA using reverse transcriptase
3) transform a bacteria and select for appropriate antibiotic resistance
4) insert the cDNA into a cloning vector to form recombinant DNA

• A) 1, 2, 4, 3
• B) 1, 2, 3, 4
• C) 1, 4, 2, 3
• D) 1, 3, 2, 4

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