Which of the Following Is in the Correct Order for One

question 57

Multiple Choice

Which of the following is in the correct order for one cycle of polymerase chain reaction (PCR) ?

• A)Denature DNA; add fresh enzyme; anneal primers; add dNTPs; extend primers.
• B)Anneal primers; denature DNA; extend primers.
• C)Extend primers; anneal primers; denature DNA.
• D)Denature DNA; anneal primers; extend primers.
• E)Denature DNA; add fresh enzyme; add dNTPs; anneal primers; extend primers.

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Definitions:

Dienophile

A compound with an electron-deficient double or triple bond that readily reacts with a diene in a Diels-Alder reaction.

Major Organic Product

The most abundant product formed in a chemical reaction involving organic compounds, often favored kinetically or thermodynamically.

Major Organic Product

The primary product resulting from an organic reaction, typically formed in the highest yield under the reaction conditions.

Major Organic Product

The superior product that emerges with the greatest efficiency from an organic chemical reaction.