Which of the Following Statements Is Correct When Comparing Two

question 23

Multiple Choice

Which of the following statements is correct when comparing two common sequencing techniques,the chain termination method and next generation sequencing?

• A)The chain termination method is faster and more efficient, so it is used to generate large-scale sequences, while next generation synthesis is used for routine, small-scale jobs.
• B)The chain termination method employs the polymerase chain reaction, but next generation sequencing does not.
• C)In the chain termination method, the order of bases is detected by fluorescently labeling each dideoxynucleotide in a different color, while next generation sequencing determines the order of bases by detecting the release of PPi during the formation of the phosphodiester bond.
• D)Next generation sequencing employs electrophoresis, but the chain termination method does not.
• E)Next generation sequencing moves a single strand of DNA through a very long pore, but the chain termination method does not.

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