Explain the Principles Underlying the Use of Doubly Labeled Water

question 11

Essay

Explain the principles underlying the use of doubly labeled water to assess total energy expenditure.Include a discussion of the main source of error.

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Definitions:

Chargaff's Rules

Empirical rules stating that DNA from any cell of any organisms has a 1:1 ratio of pyrimidine and purine bases (cytosine to guanine and adenine to thymine).

Purines

Nitrogenous bases found in DNA and RNA that include adenine and guanine.

Pyrimidines

One of the two types of nitrogenous bases found in nucleic acids, characterized by a single ring structure, and includes cytosine, thymine (DNA only), and uracil (RNA only).

Semiconservative Replication

The process by which DNA duplicates itself, where each new DNA molecule consists of one old strand and one newly synthesized strand.