A Diagram of the Mutagenesis and Crossing Strategy Used by Nüsslein-Volhard

question 8

Multiple Choice

A diagram of the mutagenesis and crossing strategy used by Nüsslein-Volhard and Wieschaus to generate and maintain segmentation mutants is shown. Male flies were exposed to EMS (a mutagen) and mated to a non-mutagenized female. How did the researchers ensure that the offspring of Cross B (i.e., the offspring seen in generation C in the diagram) were heterozygotes that carried a mutagenized chromosome? Please select all that apply.

• A) CyO homozygotes die
• B) Flies homozygous for segmentation mutations usually die
• C) Only male flies were mutagenized
• D) The female parent in Cross B carries a temperature-sensitive lethal allele

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Carrying Cost

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