Exhibit 2B
Contains Information on the PK's of Some Common

question 74

Multiple Choice

Exhibit 2B
Contains information on the pK's of some common buffers.
$\begin{array}{llrc}\text { Buffer } & \mathbf{p K} 1 & \mathbf{p K 2} & \mathbf{p K} 3 \\\hline \text { Acetate } & 4.75 & & \\\text { Ammonia } & 9.25 & & \\\text { Carbonic acid } & 6.37 & 10.20 & \\\text { Citric acid } & 3.09 & 4.75 & 5.41 \\\text { Formic Acid } & 3.75 & & \\\text { Phosphoric acid } & 2.14 & 7.20 & 12.4 \\\text { Pyruvic acid } & 2.50 & & \\\text { Tris } & 8.3 & &\end{array}$

-Refer to Exhibit 2B. The enzyme lysozyme has an optimum pH close to 5. A suitable buffer would be:

• A) Acetate
• B) Carbonate
• C) Phosphate
• D) Pyruvate
• E) None of these is a suitable buffer for this reaction.

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Definitions:

Standard Deviation

A gauge of the extent to which a collection of numbers varies or is spread out.

Mean Weight

The average weight of a set of objects or individuals, calculated by summing their weights and dividing by the number of objects or individuals.

Standard Deviation

It quantifies the amount of variation or dispersion of a set of data values from its mean, serving as an indicator of the data's spread.

Sobriety

The state of being sober, which can refer to abstinence from alcohol and drugs, or having a clear, unaffected mind.