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Exhibit 2B
Contains Information on the PK's of Some Common

question 59

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Exhibit 2B
Contains information on the pK's of some common buffers.
 Buffer pK1pK2pK3 Acetate 4.75 Ammonia 9.25 Carbonic acid 6.3710.20 Citric acid 3.094.755.41 Formic Acid 3.75 Phosphoric acid 2.147.2012.4 Pyruvic acid 2.50 Tris 8.3\begin{array}{llrc}\text { Buffer } & \mathbf{p K} 1 & \mathbf{p K 2} & \mathbf{p K} 3 \\\hline \text { Acetate } & 4.75 & & \\\text { Ammonia } & 9.25 & & \\\text { Carbonic acid } & 6.37 & 10.20 & \\\text { Citric acid } & 3.09 & 4.75 & 5.41 \\\text { Formic Acid } & 3.75 & & \\\text { Phosphoric acid } & 2.14 & 7.20 & 12.4 \\\text { Pyruvic acid } & 2.50 & & \\\text { Tris } & 8.3 & &\end{array}

-Refer to Exhibit 2B. A phosphate buffer would work well at this pH:


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