Exhibit 2B
Contains Information on the PK's of Some Common

question 74

Multiple Choice

Exhibit 2B
Contains information on the pK's of some common buffers.
$\begin{array}{llrc}\text { Buffer } & \mathbf{p K} 1 & \mathbf{p K 2} & \mathbf{p K} 3 \\\hline \text { Acetate } & 4.75 & & \\\text { Ammonia } & 9.25 & & \\\text { Carbonic acid } & 6.37 & 10.20 & \\\text { Citric acid } & 3.09 & 4.75 & 5.41 \\\text { Formic Acid } & 3.75 & & \\\text { Phosphoric acid } & 2.14 & 7.20 & 12.4 \\\text { Pyruvic acid } & 2.50 & & \\\text { Tris } & 8.3 & &\end{array}$

-Refer to Exhibit 2B. The enzyme lysozyme has an optimum pH close to 5. A suitable buffer would be:

• A) Acetate
• B) Carbonate
• C) Phosphate
• D) Pyruvate
• E) None of these is a suitable buffer for this reaction.

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