Differential Centrifugation at Low Speeds (Several Thousand RPM) Is a Useful

question 46

Multiple Choice

Differential centrifugation at low speeds (several thousand RPM) is a useful step when

• A) organelles have been lysed.
• B) enzymes of interest have different sizes.
• C) cell membranes must be left intact.
• D) ribosomes need to be broken down.
• E) there are either organelles or debris to separate.

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Definitions:

Anaphase

Stage of mitosis in which the chromosomes move to opposite poles of the cell; anaphase occurs after metaphase and before telophase.

Centromere

A specialized constricted region of a chromatid; contains the kinetochore. In cells at prophase and metaphase, sister chromatids are joined in the vicinity of their centromeres.

Nucleosomes

Structural units of a chromosome, consisting of a segment of DNA wound around a core of histone proteins.

Histone Proteins

Proteins found in the nucleus of eukaryotic cells that package and order the DNA into structural units called nucleosomes.