I Find That Briefly Washing Cells in a Buffered Solution

question 73

Multiple Choice

I find that briefly washing cells in a buffered solution at pH 11 is effective for isolating a cell membrane-associated hormone receptor. (A standard growth solution for the cells is pH 7.) Which of following best fits this description?

• A) This is an integral membrane protein that completely traverses the cell membrane.
• B) This is an integral membrane protein that only penetrates one leaf of the cell membrane.
• C) This is a peripheral membrane protein that probably interacts with membrane phospholipids using aspartate-mediated salt bridges.
• D) This is a peripheral membrane protein that probably interacts with membrane phospholipids using lysine-mediated salt bridges.

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