Exhibit 13A This Is a Map of the PBR322 Plasmid

question 11

Multiple Choice

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located.

-Refer to Exhibit 13A. If a recombinant plasmid was obtained inserting DNA into the BamHI site, screening for the recombinant plasmid can be done by the following technique.

• A) Plate on nutrient agar plates that contain ampicillin.
• B) Plate on nutrient agar plates that contain tetracycline.
• C) Plate on nutrient agar plates that contain both ampicillin and tetracycline.
• D) Plate on nutrient agar plates that contain ampicillin, followed by replica plating on tetracycline.
• E) Plate on nutrient agar plates that contain tetracycline, followed by replica plating on ampicillin.

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Definitions:

Spinout Failure

A condition where a wheel spins freely without traction, potentially leading to loss of control or damage to the vehicle.

Power Divider

Interaxle differential gearing used in tandem drive, highway tractors to split torque between the two drive axles. Also known as interaxle differential.

Broken Gear Teeth

Refers to damage characterized by one or more teeth of a gear being fractured or chipped off, often leading to malfunction or failure of gear mechanisms.

Rebuilt/Exchange Unit

Refurbished equipment or components that have been restored to working order and are often exchanged for a non-functioning unit.