Exhibit 13A This Is a Map of the PBR322 Plasmid

question 61

Multiple Choice

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located.

-Refer to Exhibit 13A. If the region between the 2 sites for HgiEII were deleted, the resulting plasmid

• A) would be useless as it would lack an origin of replication.
• B) would work well for cloning.
• C) would be about 2.6 kb in length.
• D) would confer resistance to ampicillin but not tetracycline.

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Definitions:

Haploid Number

The number of chromosomes in a gamete (sex cell), representing one complete set of chromosomes from an organism.

Mitotic Spindle

An architecture made up of microtubules that divides chromosomes between two daughter cells during the process of mitosis.

Prometaphase

A stage of mitosis where the nuclear envelope breaks down and microtubules attach to kinetochores of chromosomes.

Haploid Number

The number of chromosomes in a gamete of an organism, representing one complete set of chromosomes.