The Following Steps Are All Involved in Genetic Recombination

question 95

Multiple Choice

The following steps are all involved in genetic recombination:
1) Screening for cells that contain the recombined gene.
2) Cutting the vector with restriction enzyme.
3) Mixing the gene of interest with the vector.
4) Isolating the gene of interest from its original source.
5) Ligating the gene of interest and the vector together.
The following sequence of these five steps would be typical:

• A) 1 → 2 → 3 → 4 → 5
• B) 2 → 3 → 5 → 1 → 4
• C) 5 → 4 → 3 → 2 → 1
• D) 4 → 2 → 3 → 5 → 1
• E) 2 → 3 → 5 → 4 → 1

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Definitions:

Solid-Phase Method

A technique in chemistry for synthesizing compounds where reactions are carried out on a solid support or resin, facilitating purification and separation processes.

Merrifield

Related to Robert Bruce Merrifield, an American biochemist known for developing the solid-phase synthesis of peptides, a groundbreaking technique in biochemistry.

Globular

Referring to a compact, spherical shape, often used to describe the three-dimensional structure of proteins or other macromolecules.

Fibrous

Describes materials made of or resembling fibers, often characterized by their long, thin shape and considerable strength.