Exhibit 13A This Is a Map of the PBR322 Plasmid

question 91

Multiple Choice

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located.

-Refer to Exhibit 13A. If a recombinant plasmid were obtained by inserting DNA into the EcoRV site, and the protein corresponding to the recombinant gene were expressed, which of the following statements would be false?

• A) The protein will probably contain some extra amino acids on its N-terminal.
• B) The plasmid will be resistant to ampicillin.
• C) The plasmid will not be able to replicate autonomously.
• D) The protein is not likely to be biologically active without some further treatment.
• E) The question cannot be answered, since it is unlikely that the protein will be expressed at all, because there is no promoter for mRNA synthesis.

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Definitions:

Punishment

The imposition of a penalty as a consequence of an undesirable behavior, aimed at reducing or eliminating that behavior.

Changing

The act or process of making or becoming different, often used in the context of personal, organizational, or social transformation.

Unfreezing

The initial stage in the change process where existing patterns or behaviors are challenged in preparation for change.

Refreezing

The process of stabilizing a group, organization, or system after a change has been implemented, ensuring the new state is maintained.