Exhibit 13A This Is a Map of the PBR322 Plasmid

question 86

Multiple Choice

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located.

-Refer to Exhibit 13A. If a recombinant plasmid were obtained inserting DNA into the AflIII site, the recombinant plasmid would lack which of the following properties?

• A) It can replicate autonomously.
• B) It will be resistant to the antibiotic tetracycline.
• C) It will be resistant to the antibiotic ampicillin.
• D) It will possess at lest two promoter sites.
• E) All of these are true for the recombined plasmid.

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Definitions:

Exotic Species

Non-native species introduced to an area where they do not naturally occur, often leading to environmental imbalance.

Overexploitation

Refers to the excessive use of natural resources, leading to their depletion or significant decline.

Depletion

Depletion refers to the decrease in the quantity or quality of a resource, such as natural resources, leading to diminished value or utility.

Wild Species

Species that occur naturally in the wild and are not domesticated or cultivated.