Exhibit 13A This Is a Map of the PBR322 Plasmid

question 86

Multiple Choice

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located.

-Refer to Exhibit 13A. If a recombinant plasmid were obtained inserting DNA into the AflIII site, the recombinant plasmid would lack which of the following properties?

• A) It can replicate autonomously.
• B) It will be resistant to the antibiotic tetracycline.
• C) It will be resistant to the antibiotic ampicillin.
• D) It will possess at lest two promoter sites.
• E) All of these are true for the recombined plasmid.

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