Your Lab Uses the BioRad HPLC System for Hemoglobin Analysis

question 19

Multiple Choice

Your lab uses the BioRad HPLC system for hemoglobin analysis.As you set up for the analysis, you power up the instrument to allow it to reach operational temperature and prepare the mobile phase buffer.When the instrument and mobile phase are ready, you begin pumping the mobile phase through the system to allow the instrument to equilibrate.You next inject your calibrator.The resulting chromatogram shows nothing but a flat baseline.You inject a larger volume of calibrator and get the same result.Which of the following would explain these results?

• A)The column heater is not set too high.
• B)The detector is set at the wrong wavelength.
• C)Chromatographic windows are not assigned properly.
• D)The mobile phase was not prepared to the correct ionic strength.

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