Isoelectric Focusing (IEF) May Be Utilized to Investigate Unresolved HPLC

question 6

Multiple Choice

Isoelectric focusing (IEF) may be utilized to investigate unresolved HPLC hemoglobin results.What advantage of this technique makes it useful in this situation?

• A)IEF is a technique that is readily adaptable to automation, resulting in a quick and simple method to resolve questionable HPLC hemoglobin results.
• B)IEF is a technique that does not depend on the charge of the hemoglobin fractions, providing a method that complements HPLC analysis of hemoglobin fractions.
• C)IEF results in the formation of discrete sharp bands, allowing clear separation of the various hemoglobins present in the patient sample.
• D)IEF provides an enhanced method of detection that may allow the technologist to see fractions not detected by HPLC.

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