Consider the Separation of Hb 1, Hb 2, and Hb

question 14

Multiple Choice

Consider the separation of Hb 1, Hb 2, and Hb 3 by cation-exchange HPLC.The initial mobile phase pH causes Hb 1 to have the greatest positive charge, Hb 2 to have minimal positive charge, and Hb 3 to be neutral.Predict the order of elution from the stationary phase as the chromatographic run continues from the initial mobile phase to mobile phases with increasing concentrations of cations.(first to elute to last to elute)

• A)Hb 1, Hb 2, Hb 3
• B)Hb 1, Hb 3, Hb 2
• C)Hb 2, Hb 1, Hb 3
• D)Hb 3, Hb 2, Hb 1

---

Definitions:

Muscle Contraction

The process where muscle fibers shorten or lengthen to produce movement or maintain posture.

Contraction Cycle

The series of events in muscle fibers that lead to shortening and generating force, crucial for movement.

Myofibril

A basic rod-like unit of a muscle cell, composed of long proteins including actin, myosin, and others, responsible for muscle contraction and relaxation.

Muscle Fiber

A single muscle cell, typically cylindrical in shape, that contracts in response to stimulation and is essential for producing bodily movements.