Quantification of Hb A2 Following Separation by Cation-Exchange HPLC Produced

question 4

Multiple Choice

Quantification of Hb A2 following separation by cation-exchange HPLC produced a result of 15% in an Hb AA patient.Which of the following describes a concern the technologist should have regarding this result?

• A)There may be other hemoglobin variants co-eluting with Hb A2, causing the Hb A2 result to be falsely elevated.
• B)The low Hb A2 result may be falsely decreased because of an integration error caused by a rising baseline.
• C)Quantification of Hb A2 by HPLC is no longer recognized by CAP because of significant intralaboratory variation in Hb A2 measurements.
• D)Glycation of Hb A2 may cause its concentration to vary significantly, based on the extent to which Hb A2 is glycated.

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