Suppose HPLC Results Suggest Hb S Trait

question 17

Multiple Choice

Suppose HPLC results suggest Hb S trait.Why should this be confirmed with the sickle solubility test?

• A)Retention characteristics of hemoglobins vary significantly between chromatographic runs, requiring this confirmation step.
• B)With chromatography, there is always the possibility of unknown hemoglobins co-eluting with the suspected hemoglobin.
• C)The use of chromatographic windows has been associated with a high degree of imprecision.
• D)The sickle solubility test is the only technique available to quantify Hb S.

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