If the Ionic Strength of the Electrophoretic Buffer Is Increased

question 6

Multiple Choice

If the ionic strength of the electrophoretic buffer is increased from 0.1 M to 1.0 M and the system is operated at the same fixed voltage when using each buffer concentration, which of the following describes the results that would likely be seen when using the 1.0 M buffer, as compared to the results seen with the 0.1 M buffer?

• A)Electrophoretic separation would be achieved in shorter time with the 1.0 M buffer, with greater resolution.
• B)The electrophoretic pattern would be distorted, and the macromolecules may be denatured when using the 1.0 M buffer.
• C)Electrophoretic separation would be achieved in shorter time with the 1.0 M buffer, with decreased resolution.
• D)The electrophoretic separation time would have to be increased 10-fold to account for the 10-fold increase in buffer concentration.

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