In Radial Immunodiffusion, Calibrators of Known Concentration Are Analyzed Along

question 19

Multiple Choice

In radial immunodiffusion, calibrators of known concentration are analyzed along with the patient sample to allow for the quantification of antigens as illustrated in Figure 7-9 in your text book.Suppose the diameter of the precipitation ring for the highest calibrator was less than the diameter for the next highest calibrator.Which of the following statements describes the likely cause for this problem?

• A)The incubation temperature was not kept constant.
• B)The concentration of the antibody in the gel was too high for the concentration of the highest calibrator.
• C)Because the concentration of the highest calibrator is so high, diffusion through the pores of the gel was restricted.
• D)The concentration of the highest calibrator was greater than the concentration of the antibody in the gel.

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