The Mutation-Detection Technique Designed to Amplify Only One of the Alleles

question 13

Multiple Choice

The mutation-detection technique designed to amplify only one of the alleles present at the locus of interest by the use of primers that are substantially mismatched at one allele but have sufficient complementarity to anneal to and amplify the other allele is known as:

• A)PCR-mediated site-directed mutagenesis (PSM) technique
• B)amplification refractory mutation system (ARMS) technique
• C)allele-specific oligonucleotide hybridization (ASO or dot blot) technique
• D)reverse dot blot technique