Fluorescent Probes That Hybridize to PCR Products During Amplification Change

question 6

Multiple Choice

Fluorescent probes that hybridize to PCR products during amplification change fluorescence by two mechanisms._____ probes change fluorescence by breaking covalent bonds between the probes, and _____ probes change fluorescence based on the proximity of the two probes.Melting curve profiles are only possible with _____ probes.

• A)TaqMan, FRET, TaqMan
• B)TaqMan, FRET, FRET
• C)FRET, TaqMan, TaqMan
• D)FRET, TaqMan, FRET

---

Definitions:

Oxidative Phosphorylation

The production of ATP using energy derived from the transfer of electrons in the electron transport system of mitochondria; occurs by chemiosmosis.

Electron Transport Chain

A series of protein complexes and small organic molecules within cell membranes that transfer electrons, driving the synthesis of ATP.

ATP Synthesis

The process of creating adenosine triphosphate (ATP), the cellular energy currency, primarily through cellular respiration in mitochondria or photosynthesis in chloroplasts.

Anaerobic Respiration

A form of respiration where energy is produced in the absence of oxygen, utilizing other electron acceptors and producing different end products.