The Following Laboratory Data Were Collected Utilizing a Chemical Reaction

question 6

Multiple Choice

The following laboratory data were collected utilizing a chemical reaction to measure the concentration of estrogen in a patient's urine sample.Given the wavelength of maximum absorbance for the assay is 525 nm, and the background is known to be linear between 490 nm and 560 nm, calculate the concentration of estrogen in the patient sample using the Allen correction.
Patient sample absorbance at 490 nm = 0.298
Patient sample absorbance at 525 nm = 0.405
Patient sample absorbance at 560 nm = 0.140
Standard absorbance at 490 nm = 0.180
Standard absorbance at 525 nm = 0.492
Standard absorbance at 560 nm = 0.036
Standard concentration = 25 mg/L

• A)12.1 mg/L
• B)24.0 mg/L
• C)26.0 mg/L
• D)51.6 mg/L

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Definitions:

Reagents

Chemical substances used in reactions to bring about and probe chemical changes, often to transform reactants into products.

2,2-dimethyloxirane

A type of oxirane with two methyl groups attached to the same carbon atom, used in organic synthesis.

Ethanol

A volatile, flammable, colorless liquid alcohol, used as a solvent, antiseptic, and intoxicant.

Trace of HCl

A very small amount of Hydrochloric acid, often used in chemical reactions.