Porphyrias Are Characterized by Reduced Activity or a Deficiency of a Specific

question 9

Multiple Choice

Porphyrias are characterized by reduced activity or a deficiency of a specific enzyme.If methods were available to accurately measure activity of each enzyme in the heme synthetic pathway, why would the red blood cell not be a good place to quantify enzyme activity?

• A)Red blood cells do not carry out heme synthesis.
• B)Approximately half of heme synthesis in the red blood cell occurs within the mitochondrion, which is lost as these cells mature.
• C)Enzyme assays cannot be performed on enzymes that are isolated with red blood cells.
• D)Red blood cells must be hemolyzed to allow enzyme measurement, which would produce a spectral interference.

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