Use the Following Information When Answering the Corresponding Question(s)

question 33

Multiple Choice

Use the following information when answering the corresponding question(s) .
The lac operon is used in countless ways for genetics research and biotechnology. One important use of the lac operon is to regulate the expression of cloned genes. In this application, some regulatory elements of the operon are fused to the cloned gene that is to be regulated. One application of such technology was reported by Cronin et al., The lac operator-repressor system is functional in the mouse, Genes and Development 15 (2001) :1506-17. These investigators fused a portion of the lac operon to the mouse tyrosinase gene, a gene required for pigment production. This recombinant DNA, composed of E. coli lac operon sequences and the mouse tyrosinase gene, was introduced into albino mice using techniques described in Chapter 19. Using the lac operon regulatory sequences, the investigators were able to regulate tyrosinase gene expression. In fact, they were able to convert albino (white) mice into brown mice. The following questions ask you to use your knowledge of the lac operon to deduce how Cronin et al. were able to regulate tyrosinase gene expression in mice.
-The tyrosinase gene used by Cronin et al.contained the protein-coding sequence and the normal mouse regulatory sequences.These regulatory sequences provide positive control of tyrosinase transcription.If nothing else is done to this DNA and it is introduced into a mouse,tyrosinase will always be expressed.Because the goal was to regulate expression of tyrosinase,what portion of the lac operon do you think was added to this mouse gene?

• A)the lacZ gene
• B)the lacY gene
• C)the lacI gene
• D)the lac operator
• E)CAP

Understand the purpose and types of inventories, including work-in-process and maintenance, repair, and operations (MRO) inventories.

Comprehend the importance of cycle counting, its implementation, and how it varies from item to item based on classification.

Identify the fundamental issues in inventory management, including selection of items for order and choice of suppliers.

Grasp the concept and application of ABC analysis in inventory management for prioritizing inventory control efforts.

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Definitions:

Units of Production

A method of depreciation that calculates an asset's expense based on its usage or output, rather than a standard time period.

Time

Time is a dimension in which events occur in sequence from the past through the present to the future, and it is a measure of durations and intervals.

Dollars

The unit of currency used in the United States and other countries, often symbolized by $.

Internal Rewards

Personal satisfaction and intrinsic motivations that come from within an individual, rather than external rewards.