What Is a Primary Difference Between PCR and Traditional Cloning

question 21

Multiple Choice

What is a primary difference between PCR and traditional cloning procedures such as those used to clone the human growth hormone gene?

• A)PCR and traditional cloning make use of different types of bacteria.
• B)PCR and traditional cloning make use of different types of vectors.
• C)PCR uses plasmid vectors,whereas traditional cloning uses bacteria.
• D)PCR eliminates the need for restriction enzymes,vectors,and cells.
• E)PCR is more time-consuming,but the purity of the obtained DNA clone is much higher than in traditional cloning.

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Definitions:

Electrochemical Gradient

The combined difference in concentration and charge of ions across a membrane, driving the movement of ions through channels, critical for processes like muscle contraction and nerve transmission.

ATP

Adenosine triphosphate, a molecule that carries energy within cells for metabolism.

Chemiosmosis

The movement of ions across a selectively permeable membrane, down their electrochemical gradient, which is used in cellular respiration and photosynthesis to generate ATP.

Oxidative Phosphorylation

A metabolic process that produces ATP by transferring electrons from NADH and FADH2 to oxygen via a series of electron carriers.