Since Dideoxy Sequencing Is Based on the Chain Termination,why Are

question 9

Multiple Choice

Since dideoxy sequencing is based on the chain termination,why are normal deoxynucleotides also included in the reaction?

• A)to enhance the chain termination ability of the deoxynucleotides
• B)to provide a substrate for DNA polymerase
• C)to produce a range of DNA synthesis products that terminate at every occurrence of a particular base
• D)to create DNA synthesis products long enough to allow running a gel

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