You Ask Your Student to Transfer a Plasmid-Free Bacterial Strain

question 28

Multiple Choice

You ask your student to transfer a plasmid-free bacterial strain to multiple small tubes for long term storage.A short while later,your student admits that he did not use a clean sterile pipette to transfer the bacteria to the small tubes,but rather grabbed the same pipette he had just used to collect newly transformed bacteria.Why would this pose a problem?

• A)Plasmids can be transferred between bacteria via pili.
• B)Newly transformed bacteria will actively undergo binary fission.
• C)Plasmid can contain genes that confer antibiotic resistance.
• D)Plasmids will incorporate into the chromosomal DNA.

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Definitions:

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Referring to something occurring within the skull.

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