Which of the Following Regarding the Hydrolysis of P-Nitrophenyl Acetate

question 22

Multiple Choice

Which of the following regarding the hydrolysis of p-nitrophenyl acetate by chymotrypsin is correct?

• A)The active site contains a serine residue,an unprotonated histidine,and an unprotonated aspartate residue at pH 7.0 prior to binding the substrate.
• B)The first product is acetic acid.
• C)An actyl-enzyme tetrahedral intermediate forms during the mechanism.
• D)Attack of a water molecule on the acyl-enzyme intermediate yields the first product.

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