Which of the Following Is the Best Procedure in Order

question 7

Multiple Choice

Which of the following is the best procedure in order to position a gene in an expression vector downstream from a promoter (i.e.,perform directional cloning) ?

• A)Use a vector with 2 sites for the same restriction endonuclease.
• B)Treat the gene with DNase I.
• C)Restrict both the gene and the plasmid with 1 restriction endonuclease,and then screen all colonies for those that express the gene.
• D)Restrict each end of the DNA fragment (gene)with a different restriction endonuclease,and do likewise for the plasmid.

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