Which of the Following Is a Method Used to Insert

question 4

Multiple Choice

Which of the following is a method used to insert or transform cells with a plasmid?

• A)add the DNA to bacterial cells that have been lightly treated with lysozyme to produce "holes" in the cell wall
• B)add the DNA to a heated suspension of cells at 42°C
• C)treat the bacteria with Ca²⁺,add the DNA,and briefly heat to 42°C
• D)incubate the DNA with the cells overnight at 4°C

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Definitions:

Attentiveness

The quality of paying close and continuous attention to something or someone.

Younger Infants

Infants typically ranging from birth to 12 months old, during which rapid physical and cognitive development occurs.

Older Infants

Infants typically between the ages of 9 to 12 months who are developing new skills such as crawling, standing, or beginning to walk.

Habituate

The process of becoming accustomed to a stimulus over time, leading to a decrease in one's response to that stimulus.