In the Southern Hybridization Procedure,after Electrophoresis the Gel Is Treated

question 23

Multiple Choice

In the Southern hybridization procedure,after electrophoresis the gel is treated with NaOH and then neutralized before blotting.What is the primary function of the alkaline treatment?

• A)to cleave the DNA into smaller fragments to permit greater efficiency of transfer
• B)to inactivate any restriction endonucleases that may be in the gel
• C)to denature the duplex DNA to single-stranded DNA (ssDNA)
• D)to neutralize any acidic phosphate groups that might prevent hybridization

Understand the distinction between quantitative and qualitative research methods.

Acknowledge the role of empirical evidence in scientific research.

Understand the basics of Maslow's hierarchy of needs and its application in real-life scenarios.

Comprehend the role of the lateral hypothalamus and its relation to eating behavior.

---

Definitions:

Inventory Destroyed

Refers to stock items that have been damaged, expired, or cannot be sold, leading to a loss for the business.

Typical Gross Profit

The average gross profit a company makes after subtracting the cost of goods sold from its net sales.

FOB Shipping

Free On Board Shipping, a term used in shipping agreements to specify which party (buyer or seller) pays for shipping costs and when the ownership of the goods transfers.

Import Duties

Import duties are taxes imposed by a government on goods imported into a country, often used to protect domestic industries and raise revenue.