Which of the Following Is the Best Procedure in Order

question 7

Multiple Choice

Which of the following is the best procedure in order to position a gene in an expression vector downstream from a promoter (i.e.,perform directional cloning) ?

• A)Use a vector with 2 sites for the same restriction endonuclease.
• B)Treat the gene with DNase I.
• C)Restrict both the gene and the plasmid with 1 restriction endonuclease,and then screen all colonies for those that express the gene.
• D)Restrict each end of the DNA fragment (gene)with a different restriction endonuclease,and do likewise for the plasmid.

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Definitions:

Absolute Advantage

The ability of an individual, company, or country to produce a good or service at a lower cost per unit than others.

Comparative Advantage

An economic principle stating that countries or entities gain and benefit from trade when they specialize in producing goods for which they have a lower opportunity cost than their trading partners.

Production

The process of combining various material inputs and immaterial inputs (plans, know-how) in order to make something for consumption (the output).

Producer Surplus

The difference between what producers are willing to accept for a good or service versus what they actually receive in the market.