Which of the Following Steps Is NOT Involved with Each

question 16

Multiple Choice

Which of the following steps is NOT involved with each cycle of amplification in PCR?

• A)the addition of fresh dNTPs to the reaction mixture
• B)annealing of oligodeoxyribonucleotide primers to DNA
• C)thermal denaturation of the target duplex DNA
• D)reaction with DNA polymerase at approximately 70°C

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