The Figure Below Diagrams a Process Used to Study Bacterial

question 8

Multiple Choice

The figure below diagrams a process used to study bacterial physiology. Which is the correct description of the steps shown?

• A) Cells are lysed, two-dimensional gels are run, and then spots of protein are run through mass spec. Proteins' peaks are identified.
• B) Cells are lysed, two-dimensional gels are run, isobaric tags are added to the proteins, and they are run through a mass spec. Proteins' peaks are identified.
• C) Cells are lysed, two-dimensional gels are run, and proteins are digested with trypsin and run through a mass spec. Proteins' peaks are identified.
• D) Cells are lysed, proteins are digested with trypsin, and column is used followed by mass spec. Proteins' peaks are identified.
• E) Cells are lysed, RNA is purified, cDNA is made, and sequencing is carried out.

---

Definitions:

Converts

Individuals who adopt a new religious faith different from their previous belief or non-belief.

Environmental Scientist

A professional who studies and evaluates the impact of human activities on the environment, working towards sustainable solutions.

Rachel Carson

Rachel Carson was an American marine biologist, author, and conservationist whose book "Silent Spring" and other writings are credited with advancing the global environmental movement.

Emotional Woman

A stereotypical and problematic representation of women as being more prone to emotional responses than rational decision-making, often used to undermine their credibility.