The Figure Below Diagrams a Process Used to Study Bacterial

question 8

Multiple Choice

The figure below diagrams a process used to study bacterial physiology. Which is the correct description of the steps shown?

• A) Cells are lysed, two-dimensional gels are run, and then spots of protein are run through mass spec. Proteins' peaks are identified.
• B) Cells are lysed, two-dimensional gels are run, isobaric tags are added to the proteins, and they are run through a mass spec. Proteins' peaks are identified.
• C) Cells are lysed, two-dimensional gels are run, and proteins are digested with trypsin and run through a mass spec. Proteins' peaks are identified.
• D) Cells are lysed, proteins are digested with trypsin, and column is used followed by mass spec. Proteins' peaks are identified.
• E) Cells are lysed, RNA is purified, cDNA is made, and sequencing is carried out.

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