His₆-Fusion-Tagged Proteins Can Be Recovered from Affinity Chromatography Columns Because

question 3

Multiple Choice

His₆-fusion-tagged proteins can be recovered from affinity chromatography columns because the histidine tag tightly binds to beads coated with ________ ions. The elution buffer contains ________, which has stronger affinity for these ions.

• A) copper; sulfate
• B) magnesium; EDTA
• C) iron; citrate
• D) calcium; EGTA
• E) nickel; imidazole

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Definitions:

Scatterplot

Graphical display of paired scores on two variables measured at the interval or ratio level of measurement.

Variables

Attributes, characteristics, or conditions that can change or vary within or across subjects in a study.

Prediction

An educated guess about future events based on current knowledge, data, or trends.

Predictor Variables

Variables that are used in statistical models to predict or explain variations in another variable, often the dependent variable.