How Is Bacterial DNA Cross-Linked to Putative Binding Proteins for Chromatin

question 48

Multiple Choice

How is bacterial DNA cross-linked to putative binding proteins for chromatin immunoprecipitation (ChIP) ?

• A) exposure to UV light
• B) treatment with formaldehyde
• C) treatment with heat
• D) reduction with dithiothreitol
• E) reduction with b-mercaptoethanol

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Definitions:

Lipoproteins

Molecules made of fat and protein that transport lipids (fats) in the blood.

Cholesterol

A waxy, fat-like substance found in all cells of the body, necessary for making hormones, vitamin D, and substances that help digest foods.

LDL

Low-Density Lipoprotein, often referred to as "bad" cholesterol, which can lead to plaque buildup in arteries, increasing the risk of heart disease.

Peptidases

Enzymes that break down peptides into their component amino acids by cleaving the peptide bonds between them.