Real-Time PCR Can Be Used to Quantify the Original Amount

question 50

Multiple Choice

Real-time PCR can be used to quantify the original amount of RNA in a sample. What additional step must be accomplished in this particular application?

• A) the careful design of specific forward and reverse primers
• B) RNA must first be converted to cDNA.
• C) RNA must be denatured with formaldehyde to avoid secondary structures.
• D) the separation of mRNA from other RNA species
• E) DNA is converted to RNA.

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