Each of the Following Was Used to Elucidate the TCA

question 53

Multiple Choice

Each of the following was used to elucidate the TCA cycle EXCEPT

• A) using radiolabeled acetate to determine which carbons were oxidized to CO₂ .
• B) the use of crude enzyme preparations from a variety of sources such as cucumber seeds and beef liver.
• C) adding a variety of short-chain fatty acids commonly found in cells to detect activity.
• D) the use of C14-labeled carbon compounds as a source of carbon under anoxic conditions.
• E) using C13 to determine which intermediates are enriched under various growth conditions.

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