The Restriction Enzymes BamHI and BclI Cut at the Points

question 4

Multiple Choice

The restriction enzymes BamHI and BclI cut at the points indicated by arrows:

If one sample of DNA was cut with BamHI and another with BclI,and these two samples were mixed and treated with DNA ligase,what would occur?

• A)No DNAs would be ligated (joined together).
• B)Only the BamHI-cut DNA would be ligated.
• C)Only the BclI-cut DNA would be ligated.
• D)Both BamHI-cut and BclI-cut DNAs would be ligated,but only to DNA fragments cut with the same enzyme (e.g. ,BamHI fragments would be ligated only to other BamHI fragments).
• E)Both BamHI-cut and BclI-cut DNAs would be ligated with no preference for which fragment is ligated to which other.

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