In a Single PCR Cycle Consisting of 15 Seconds at 94°C,30

question 5

Multiple Choice

In a single PCR cycle consisting of 15 seconds at 94°C,30 seconds at 50°C,and 1 min at 72°C,what is happening in the step run at 50°C?

• A)The DNA to be amplified is being denatured.
• B)Primers are being denatured.
• C)DNA polymerase is extending new DNA from the primers.
• D)Primers are annealing to the DNA to be amplified.
• E)DNA polymerase is being inactivated.

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