In the Sanger Method of DNA Sequencing,incorporation of the Nucleotides

question 35

Multiple Choice

In the Sanger method of DNA sequencing,incorporation of the nucleotides stops because

• A)the dideoxynucleotide analogs have no 3'-OH group to continue DNA synthesis.
• B)the dideoxynucleotide analogs have no 5'-OH group to continue DNA synthesis.
• C)the dideoxynucleotide analogs have no 2'-OH group to continue DNA synthesis.
• D)the mixture is heated to 92°C to stop DNA synthesis at specific time intervals.
• E)the dideoxynucleotide analogs are in greater concentration than the actual nucleotides.

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Definitions:

Planned Order Releases

Instructions to start the manufacture, ordering, or delivery of items in a specific period, in accordance with the production schedule.

Projected Output Rate

The estimated rate at which a system or process is expected to produce goods or complete tasks over a specific period.

Inventory Holding Costs

The expenses associated with storing unsold goods, including costs related to warehousing, insurance, depreciation, and opportunity cost.

Lot-For-Lot

An inventory ordering strategy that matches the exact quantity needed for production, minimizing holding costs.