In the Process of Isolating DNA for Transcription and Translation,you

question 24

Multiple Choice

In the process of isolating DNA for transcription and translation,you digest the DNA with BamHI and ligate the gene into a plasmid vector.However,even though all of the controls work,no transcription is obtained using your fragment.Working backward,you note that restriction digestion has removed approximately 250 base pairs from the 5' end of the gene.It is possible that during restriction digestion

• A)the termination signal was removed.
• B)the RNA polymerase became inactivated.
• C)primase could not bind.
• D)the promoter was removed.
• E)none of the above

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Definitions:

Occipital Lobes

The area of the brain situated at the rear of the skull, tasked with interpreting visual data.

EEG

Electroencephalography, a monitoring method to record electrical activity of the brain, used in the diagnosis of brain disorders.

Synapse

The junction between two neurons where information is transmitted from one neuron to another.

Agonist

A chemical or drug that binds to receptors in the brain and activates them, often mimicking the effects of naturally occurring substances.