You Expect That a Mutation in E

question 27

Multiple Choice

You expect that a mutation in E.coli that was recently isolated results in loss of enzyme R activity.In looking for the gene,you find comparable sequences.Using an in vitro transcription translation system,you use the gene obtained from the mutant,as well as a wild type E.coli as a control.Following protein isolation and gel electrophoresis,you observe that the wild type E.coli produces a protein that is 35K in size,corresponding to enzyme R.However,the mutant strain produces a protein that is 25K in size.What does this information suggest?

• A)A nonsense mutation has occurred, resulting in a truncated, nonfunctional product in the mutant.
• B)A large addition has occurred in the wild type strain, such that approximately 35 tandem repeats of this gene are present and are biologically active.
• C)A missense mutation has occurred, resulting in a truncated, nonfunctional product in the mutant.
• D)all of the above
• E)Not enough information is given.

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Definitions:

Industrial Average

A stock market index that measures the performance of a sector of the economy, typically composed of leading companies in that sector.

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Refers to a stock market index in which each company's stock influences the index in proportion to its price per share.

Money Market

A segment of the financial market in which short-term financial instruments, such as Treasury bills and commercial paper, are traded.

Treasury Bond

Long-term, fixed-interest U.S. government debt security with a maturity of more than 10 years.